Supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture need to be ordered separately.
Description: Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR* reactions.
Applications: Taq DNA Polymerase can be used in most applications including the following:
- 3"A-tailing of blunt ends.
- Primer extension
- DNA sequencing.
Formulation: Genscript Taq DNA Polymerase has been formulated using a proprietary technology, and the enzyme can be shipped at room temperature or stand at 37枫C for 7 days without losing any activity.
- Terminal transferase activity. Taq DNA Polymerase has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at 3" end of the extension product.
- High-purity. No contamination activity has been detected in standard test reactions.
QC Tests: PCR* performance, activity, nuclease.
Unit Definition: 1 unit of enzyme incorporates 10 nmol of dNTP into acid-insoluble material in 30 min at 74°C.
10 X reaction Buffer (with Mg2+): 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If not using this buffer, 0.1% Triton X-100 (final concentration) is a must to ensure high activity.
Storage Buffer and Concentration: Supplied in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
*The PCR process is covered by US. Patent numbers 4683195 and 4683202 issued to Cetus and owned by Hoffman-La Roche Inc. Genscript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted in regions or countries where native Taq DNA Polymerase patents have been invalidated.
DATASHEET: 20060806230259 (PDF)
MSDS: 20060807040514 (PDF)
PROTOCOL: 20061215120639 (PDF)
Publications used this GenScript Product:
- Zeyu Xin, Yihong Zhao, and Zhi-Liang Zheng. Transcriptome Analysis Reveals Specific Modulation of Abscisic Acid Signaling by ROP10 Small GTPase in Arabidopsis. Plant Physiol. Oct 2005; 139(3): 1350-1365
- Gerard Crudden, Rachel E. Chitti, and Rolf J Craven. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J. Pharmacol. Exp. Ther. Oct 2005;
- Julia C Mallory, Gerard Crudden, Amelia Oliva, Christopher Saunders, Arnold Stromberg, Rolf J Craven. A novel group of genes regulate susceptibility to anti-neoplastic drugs in highly tumorigenic breast cancer cells. Mol. Pharmacol. Sep 2005; Download Pdf
GenScript provides gene, peptide, protein, and antibody products and services to the international scientific community. GenScript specializes in custom gene and peptide synthesis, siRNA construction, and both polyclonal and monoclonal antibody production. Our state-of-the-art methodology incorporates meticulous design tools and a unified approach to synthesis that ensure our customers the highest quality results.
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